Sox Soil DNA Isolation and Purification Kit
Extract high quality DNA from microbes within a wide variety of sample types rapidly and efficiently. Our Sox Soil DNA Kit employs patent-pending methodology of bead-beating, silica spin columns, and a unique combination of chemistry, resulting in exceptionally high yields of highly purified DNA. Contaminants, including humic acids and other compounds that inhibit PCR and downstream molecular biology manipulations, are removed from the lysate. Organic solvents and proteinases are not used. This kit has been validated by successful extraction of DNA from diverse soil sample types and high-resolution 16S rRNA gene and fungal ITS profiles of DNA preps.
Why use the Sox Kit?
Isolation of high-quality genomic DNA from 250 mg of five different soil samples. For comparison, DNA was also extracted using soil DNA isolation kits from two other suppliers (M and N) according to supplier’s user guides. Eluted DNA was resolved on a 0.8% TAE agarose gel., with lambda DNA-HindIII used as a maker. MBI, Sox Soil DNA Kit (Metagenom Bio).
Bacterial 16S rRNA Profile
Fungal ITS Profile
Soil community profiles from DNA extracted using the Sox Soil DNA Kit. The protocol efficienty lyses a variety of cell types and produces inhibitor - and contaminants-free DNA, suitable for downstream molecular applications, including hight-throughput sequencing.